Cobalt Chloride Promotes Osteogenesis of Rat Bone Marrow Mesenchymal Stem Cells In Vitro and In Vivo

This work aimed to investigate the effect of low-concentration cobalt chloride on in vitro and vivo proliferation rat bone mesenchymal stem cells, expression hypoxia-inducing fraction-1 alpha related osteogenic genes. The cells were isolated from marrow rats, surface biomarkers identified by immunofluorescence staining flow cytometry. differentiation was measured alizarin red alkaline phosphatase staining. treated with at different concentrations cell viability assessed counting kit 8. Cell migration detected Transwell wound healing assay. collagen type 1, morphogenetic protein-2, runt-related transcription factor 2, quantitative real time polymerase chain reaction large segmental defect model made mimic defect. cells-collagen sponge scaffold adopted for treatment histological analysis femur structure performed assess effects chloride-treated promoting formation. X-ray micro computed tomography observe structure. cluster 90 44 highly expressed 45 relatively low cells. 300 400 μmol/l could effectively suppress proliferation, compared control group. dose (50 100 μmol/l) promote proliferation. increase positive red. hypoxiainducing statistically up-regulated treatment. chloridebone accelerated regeneration, increased new deposition protein-2 tissues. Appropriate concentration can up-regulate genes vitro, simultaneously promoted regeneration vivo.